| 非水相中脂肪酶催化甘油三酯转酯反应动力学 |
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| 引用本文： | 苏正定,姚汝华.非水相中脂肪酶催化甘油三酯转酯反应动力学[J].中国生物化学与分子生物学报,1993,9(1):42-46. |
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| 作者姓名： | 苏正定 姚汝华 |
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| 作者单位： | 华南理工大学生物工程研究所，华南理工大学生物工程研究所 广州 510641，广州 510641 |
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| 摘 要： | 本文研究了脂肪酶在己烷中催化甘油三丁酸酯与硬脂酸之间的转酯反应动力学。转酯反应分为二个连续的反应步骤:由酯生成的酰基酶复合物的水解和由酸生成的酰基酶复合物的醇解,整个反应符合乒乓反应机制,动力学参数为:K_m(酯)=3.2×10~(-2)mol/L,K_m(酸)=6.9×10~(-2)mol/L,V_m=1.7×10~(-4)mol/L·min。
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| 关 键 词： | 动力学 转酯反应 脂肪酶 非水相 |
| 收稿时间： | 1993-02-20 |
Kinetics of Lipase-Catalysed Transesterification of Triglycerides In Non-Aqenous Phase |
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| Su,Zheng-ding Yao,Ru-hua.Kinetics of Lipase-Catalysed Transesterification of Triglycerides In Non-Aqenous Phase[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(1):42-46. |
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| Authors: | Su Zheng-ding Yao Ru-hua |
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| Institution: | (Inst of Biotechn., South China Univer. of Techn., Guangchou 510641 |
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| Abstract: | This paper has reported the kinetics of transesterification catalyzed by lipase between tributyrin and stearic acid in hexane. The reaction proceeds through two acyl enzyme intermediates in two successive steps: the hydrolysis of the acyl enzyme and the alcoholysis of the acyl enzyme. The whole reaction obeys a ping pong machanism. The kinetic parameters, K_m(ester), K_m(acid) and V_m are 3.2× 10~(-2)mol/L, 6.9×10~(-2)mol/L and 1.7×10~(-4) mol/L·min respectively. |
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| Keywords: | Kinetics Transesterification Lipase Non-aqeuous phase |
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