Cyclin-dependent kinase 5 phosphorylates mammalian HMGB1 protein only if acetylated |
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Authors: | Ugrinova Iva Pashev Iliya G Pasheva Evdokia A |
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Institution: | Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamada-Oka, Suita, Japan. |
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Abstract: | Old yellow enzyme (OYE) is an NADPH oxidoreductase capable of reducing a variety of compounds. It contains flavin mononucleotide (FMN) as a prosthetic group. A ternary complex structure of OYE from Trypanosoma cruzi (TcOYE) with FMN and one of the substrates, p-hydroxybenzaldehyde, shows a striking movement around the active site upon binding of the substrate. From a structural comparison of other OYE complexed with 12-oxophytodienoate, we have constructed a complex structure with another substrate, prostaglandin H(2) (PGH(2)), to provide a proposed stereoselective reaction mechanism for the reduction of PGH(2) to prostaglandin F(2α) by TcOYE. |
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