The mitochondrial DNA helicase TWINKLE can assemble on a closed circular template and support initiation of DNA synthesis |
| |
Authors: | Jemt Elisabeth Farge Géraldine Bäckström Stefan Holmlund Teresa Gustafsson Claes M Falkenberg Maria |
| |
Affiliation: | Department of Medical Biochemistry and Cell Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden. |
| |
Abstract: | Mitochondrial DNA replication is performed by a simple machinery, containing the TWINKLE DNA helicase, a single-stranded DNA-binding protein, and the mitochondrial DNA polymerase γ. In addition, mitochondrial RNA polymerase is required for primer formation at the origins of DNA replication. TWINKLE adopts a hexameric ring-shaped structure that must load on the closed circular mtDNA genome. In other systems, a specialized helicase loader often facilitates helicase loading. We here demonstrate that TWINKLE can function without a specialized loader. We also show that the mitochondrial replication machinery can assemble on a closed circular DNA template and efficiently elongate a DNA primer in a manner that closely resembles initiation of mtDNA synthesis in vivo. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|