Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus |
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| Authors: | Ping Wu Xiaoxu Jiang Xijie Guo Long Li Tao Chen |
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| Institution: | 1Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China;2Quality Inspection Center for Sericulture Products, Ministry of Agriculture, Zhenjiang Jiangsu, China;3School of Biology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China;Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, CHINA |
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| Abstract: | Bombyx mori nucleopolyhedrosis virus (BmNPV) is a major pathogen that threatens the growth and sustainability of the sericulture industry. Since microRNAs (miRNAs) have been shown to play important roles in host-pathogen interactions, in this study we investigated the effects of BmNPV infection on silkworm microRNAs expression profile. To achieve this, we constructed and deep-sequenced two small RNA libraries generated from BmNPV infected and un-infected larvae. The results revealed that 38 silkworm miRNAs were differentially expressed after BmNPV infection. Based on the GO analysis, their predicted target genes were found to be involved in diverse functions such as binding, catalytic, virion and immune response to stimulus suggesting their potential roles in host-virus interactions. Using the dual-luciferase reporter assay, we confirmed that Bmo-miR-277-5p, up-regulated in BmNPV-infected larvae, targeted the B. mori DNA cytosine-5 methyltransferase (Dnmt2) gene which may play potential role in silkworm-BmNPV interaction. These results provide new insights into exploring the interaction mechanism between silkworm and BmNPV. |
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