Specific effect of manganese on the allantoinase of Vigna Radiata |
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Authors: | Ann Mary J. Nirmala K. Sivarama Sastry |
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Affiliation: | Department of Biochemistry, Osmania University Hyderabad 500007, A.P., India |
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Abstract: | Vigna radiata seedlings germinated in the presence of Mn2+ show an unusual increase in allantoinase activity which is proportional to Mn2+ concentration up to 5 mM. Though Mn2+ is not an activator for V. radiata allantoinase, it specifically protects allantoinase against thermal as well as papain-catalysed inactivation. Evidence is presented to show that the primary effect of Mn2+ is a protective one, both in vitro and in vivo, and that this is reflected in the observed enhancement of allantoinase activity in Mn2+ grown seedlings. That this unusual effect of Mn2+ is a specific one is indicated by the lack of a similar effect with Mg2+. Cu2+ is shown to destabilize V. radiata allantoinase in vitro as well as in vivo. |
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Keywords: | Vigna radiata Leguminosae mung bean protection of allantoinase allantoinase manganese. |
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