| 真菌激发子对人参悬浮培养细胞的生长和人参皂甙生物合成的影响 |
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| 引用本文： | 刘长军,侯嵩生.真菌激发子对人参悬浮培养细胞的生长和人参皂甙生物合成的影响[J].分子细胞生物学报,1999(2). |
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| 作者姓名： | 刘长军 侯嵩生 |
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| 作者单位： | 中国科学院上海植物生理研究所，中国科学院武汉植物研究所 上海 200032，武汉 430074 |
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| 摘 要： | 真菌诱导子处理人参悬浮培养细胞后,人参皂甙的合成有明显增加,诱导处理改变人参皂甙的积累时程,促进人参细胞培养物中次生产物的外泌,同时增强细胞对蔗糖的摄取、吸收并引起细胞H~ 流的变化。
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| 关 键 词： | 真菌诱导子 人参 细胞悬浮培养 人参皂甙 |
REGULATION OF FUNGAL ELICITORS ON THE CELL GROWTH AND BIOSYNTHESIS OF SAPONIN OF PANAX GINSENG CELL SUSPENSION CULTURE |
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| LIU Chang Jun HOU Song Sheng.REGULATION OF FUNGAL ELICITORS ON THE CELL GROWTH AND BIOSYNTHESIS OF SAPONIN OF PANAX GINSENG CELL SUSPENSION CULTURE[J].Journal of Molecular Cell Biology,1999(2). |
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| Authors: | LIU Chang Jun HOU Song Sheng |
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| Abstract: | Adding fungal elicitors to the Panax ginseng cell suspension cultures, the biosynthesis of saponin was obviously induced, the total productivity of saponin in cultures could increase more than 30% of the control. During elicitation, the accumulation patterns of saponin in suspension cultured cells were changed, the culture time for maximum biosynthesis of saponin was shortened
2-4 days comparing with that of the control, and about 80% of biosynthetic saponin in elicited cells was secreted into medium, meanwhile the uptake for sucrose in medium of cells was enhanced, and the disturbing of pH in medium was observed, which predicated that an ion exchange occurred between elicited cells and medium. |
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| Keywords: | Panax ginseng Fungal elicitor Cell suspension culture Saponin |
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