Recovery of invertase and laminarinases from industrial waste broths of baker's yeast |
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| Authors: | T. G. Villa H. J. Phaff |
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| Affiliation: | (1) Department of Food Science and Technology, University of California, 95616 Davis, California, USA;(2) Present address: Department of Microbiology, Faculty of Science, University of Salamanca, Salamanca, Spain |
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| Abstract: | Summary A procedure is described for the recovery of invertase and three laminarinases from commercial waste broths of baker's yeast. The procedure includes adsorption steps on DEAE-Sephadex A50, on concanavalin A-Sepharose 4B, and an additional gel exclusion chromatography step on Sephacryl S-200. The isolated laminarinases were: laminarinase I (exo- -D-glucanase), laminarinase II [endo--(1-3)-D-glucanase], and laminarinase III (exo--D-glucanase). The efficiency of the main concentration step (DEAE-Sephadex A50) depended on the predialysis of the crude broth. Thus, the recovery of invertase and laminarinase from nondialyzed samples was 7% and 16%, respectively. These values were increased to 62% and 100%, respectively, when the samples were previously dialyzed. |
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