Unexpected influence of ionic strength on branched-pathway interactions between beta-lactamases and beta-halogenopenicillanates. |
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| Authors: | F De Meester A Matagne G Dive and J M Frère |
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| Institution: | Laboratoires de Microbiologie et d'Enzymologie, Université de Liège, Belgium. |
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| Abstract: | Ionic strength strongly influenced the turnover/inactivation ratio in the interaction between beta-halogenopenicillanates and some class A beta-lactamases. This suggested the stabilization of a highly charged intermediate by solvation. Those data could be interpreted on the basis of a reaction pathway where an episulphonium ion was transiently formed. The various mechanisms proposed for explaining the formation of the dihydrothiazine chromophore are discussed. |
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