HLA-DQ RFLP variants of five HLA-DQw2-bearing major histocompatability complex extended haplotypes |
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| Authors: | Juan J Yunis Mark Wescott Scarlet J Lechin Sharon M Alosco Zuheir Awdeh Chester A Alper Edmond J Yunis |
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| Institution: | (1) Division of Immunogenetics, Dana-Farber Cancer Institute, Boston, Massachusetts, USA;(2) Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA;(3) American Red Cross Northeast Region, Dedham, Massachusetts, USA;(4) The Center for Blood Research, Boston, Massachusetts, USA;(5) Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA;(6) Division of Immunogenetics, Dana-Farber Cancer Institute, Dana 740, 44 Binney St., 02115 Boston, MA, USA |
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| Abstract: | We have analyzed genomic DNA in a large number of independent examples of five HLA-DQw2-bearing extended haplotypes for their associated subtypes by restriction fragment length polymorphism (RFLP) using DRB, DQA, and DQB probes after Taq I and Pst I digestion and Southern blotting. In addition to three previously described HLA-DQw2 subtypes, DQw2a, DQw2b, and DQw2c, we observed a fourth subtype, HLA-DQw2d, characterized by 5.8 kilobase (kb) DRB/Taq I, 2.4, 2.3, and 1.8 kb DQB/Taq I, and 8.0 and 2.3 kb DQA/Pst I fragments. All 22 independent examples of the extended haplotype HLA-B8,SCO1,DR3] carried DQw2a and all 11 independent examples of HLA-B18,F1C30,DR3] carried DQw2b. In addition, all independent examples (21 and 4, respectively) of two DR7-carrying extended haplotypes, HLA-B44,FC31,DR7] and HLA-Bw47,FC91,0,DR7], carried DQw2c and all independent examples of HLA-Bw57,SC61,DR7] carried DQw2d. Our results show that the DNA in the DR/DQ region of extended haplotypes is relatively fixed and that different DQw2 subtypes characterize different DQw2-bearing extended haplotypes. |
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