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Characterization of infectious hepatitis C virus from liver-derived cell lines |
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| Authors: | Akazawa Daisuke Date Tomoko Morikawa Kenichi Murayama Asako Omi Noriaki Takahashi Hitoshi Nakamura Noriko Ishii Koji Suzuki Tetsuro Mizokami Masashi Mochizuki Hidenori Wakita Takaji |
| Institution: | a Pharmaceutical Research Laboratories, Toray Industries, Inc., Kanagawa, Japan b Department of Virology II, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan c Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan |
| Abstract: | The efficient production of infectious HCV from the JFH-1 strain is restricted to the Huh7 cell line and its derivatives. However, the factors involved in this restriction are unknown. In this study, we examined the production of infectious HCV from other liver-derived cell lines, and characterized the produced viruses. Clones of the Huh7, HepG2, and IMY-N9, harboring the JFH-1 full-genomic replicon, were obtained. The supernatant of each cell clone exhibited infectivity for naïve Huh7. Each infectious supernatant was then characterized by sucrose density gradient. For all of the cell lines, the main peak of the HCV-core protein and RNA exhibited at approximately 1.15 g/mL of buoyant density. However, the supernatant from the IMY-N9 differed from that of Huh7 in the ratio of core:RNA at 1.15 g/mL and significant peaks were also observed at lower density. The virus particles produced from the different cell lines may have different characteristics. |
| Keywords: | Cell culture Hepatitis C virus Infectivity Particle Replicon |
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