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The cell growth suppressor, mir-126, targets IRS-1
Authors:Zhang Jin  Du Ying-ying  Lin Yi-feng  Chen Ya-ting  Yang Lu  Wang Hui-jun  Ma Duan
Institution:a Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical College, Fudan University, Dong’an Road 130#, Shanghai 200032, China
b Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
Abstract:miRNAs are a family of approximately 22-nuleotide-long noncoding RNAs involved in the formation and progress of tumors. Since traditional methods for the detection of miRNAs expression have many disadvantages, we developed a simple method called polyA RT PCR. With this method, we detected a series of miRNAs and found that mir-126 is one of the miRNAs underexpressed in breast cancer cells. Flow cytometry analysis showed that mir-126 inhibited cell cycle progression from G1/G0 to S. Further studies revealed that mir-126 targeted IRS-1 at the translation level. Knocking down of IRS-1 suppresses cell growth in HEK293 and breast cancer cell MCF-7, which recapitulates the effects of mir-126. In conclusion, we developed a simple method for high-throughput screening of miRNAs and found that mir-126, a cell growth suppressor, targets IRS-1.
Keywords:microRNA  mir-126  Cell growth  Target genes  IRS-1  PolyA RT PCR
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