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Thrombomodulin exerts cytoprotective effect on low-dose UVB-irradiated HaCaT cells |
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| Authors: | Iwata Masahiro Kawahara Ko-Ichi Kawabata Hisashi Ito Takashi Mera Kentaro Biswas Kamal Krishna Tancharoen Salunya Higashi Yuko Kikuchi Kiyoshi Hashiguchi Teruto Kanekura Takuro Maruyama Ikuro |
| Institution: | a Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan b Laboratory of Vascular Medicine, Department of Cardiovascular and Respiratory Disorders Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan c Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand |
| Abstract: | Thrombomodulin (TM) is an endothelial cell surface anticoagulant glycoprotein that performs antimetastatic, angiogenic, adhesive, and anti-inflammatory functions in various tissues. It is also expressed in epidermal keratinocytes. We found that a physiological dose (10 mJ/cm2) of mid-wavelength ultraviolet irradiation (UVB) significantly induced TM expression via the p38mitogen-activated protein kinase (MAPK)/cyclic AMP response element (CRE) signaling pathway in the epidermal keratinocyte cell line HaCaT; this shows that TM regulates the survival of HaCaT cells. SB203580, a p38MAPK inhibitor, significantly decreased TM expression and the viability of cells exposed to UVB. Furthermore, overexpression of TM markedly increased cell viability, and it was abrogated by TM small interfering RNA (siRNA), suggesting that TM may play an important role in exerting cytoprotective effect on epidermal keratinocytes against low-dose UVB. |
| Keywords: | TM thrombomodulin CRE cyclic AMP response element UVB irradiation mid-wavelength ultraviolet irradiation |
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