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Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice |
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| Authors: | Nishimura Takuya Sakudo Akikazu Xue Guangai Ikuta Kazuyoshi Yukawa Masayoshi Sugiura Katsuaki Onodera Takashi |
| Institution: | a Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan b Department of Virology, Center for Infectious Disease Control, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan c Laboratory of Biomedical Science, Department of Veterinary Medicine, College of Biosource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan d Food and Agricultural Materials Inspection Center, Saitama, Saitama 330-9731, Japan |
| Abstract: | Cellular prion protein (PrPC) is expressed not only in neuronal cells but also in non-neuronal cells such as astroglial cells. In the present study, the prion protein (PrP) gene (Prnp)-deficient astroglial cell line GpL1 from hippocampal cells of ZrchI Prnp−/− mice were established. Transfection of Prnp suppressed cell death in GpL1 cells under serum-free conditions. The PrP-expressing GpL1 cells showed increased superoxide dismutase activity compared to control GpL1 cells. These results suggest that the anti-oxidative activity of PrPC functions in not only neuronal cells but also astroglial cells possibly due to the increased anti-oxidative activity of astroglial cells. |
| Keywords: | Prion Immortalization Cell line |
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