Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells |
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Authors: | Thorpe S D Buckley C T Vinardell T O'Brien F J Campbell V A Kelly D J |
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Affiliation: | a Trinity Centre for Bioengineering, School of Engineering, Trinity College, Dublin, Ireland b Department of Anatomy, Royal College of Surgeons in Ireland, Ireland c Department of Physiology, School of Medicine, Trinity College, Dublin, Ireland |
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Abstract: | The objective of this study was to investigate the influence of dynamic compressive loading on chondrogenesis of mesenchymal stem cells (MSCs) in the presence of TGF-β3. Isolated porcine MSCs were suspended in 2% agarose and subjected to intermittent dynamic compression (10% strain) for a period of 42 days in a dynamic compression bioreactor. After 42 days in culture, the free-swelling specimens exhibited more intense alcian blue staining for proteoglycans, while immunohistochemical analysis revealed increased collagen type II immunoreactivity. Glycosaminoglycan (GAG) content increased with time for both free-swelling and dynamically loaded constructs, and by day 42 it was significantly higher in both the core (2.5 ± 0.21%w/w vs. 0.94 ± 0.03%w/w) and annulus (1.09 ± 0.09%w/w vs. 0.59 ± 0.08%w/w) of free-swelling constructs compared to dynamically loaded constructs. This result suggests that further optimization is required in controlling the biomechanical and/or the biochemical environment if such stimuli are to have beneficial effects in generating functional cartilaginous tissue. |
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Keywords: | Chondrogenesis Mesenchymal stem cells TGF-β3 Dynamic compression Tissue engineering |
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