(1) Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1 GA, U.K.
Abstract:
A simple modification of the TROSY pulse transfer scheme, suggested by Yang and Kay [J. Biomol. NMR 13 (1999) 3–10], is proposed which results in the suppression of unwanted anti-TROSY lines without any extra loss in sensitivity. The higher sensitivity of this TROSY transfer scheme therefore becomes available for 2D [15N, 1H] TROSY correlation and 3D/4D 15N separated NOESY type experiments where complete suppression of the broad anti-TROSY lines is essential.