| 用气相色谱法检测甲型肝炎减毒活疫苗中三氯甲烷含量 |
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| 引用本文： | 沈红杰,辛暨华,全爱顺,姜崴,张耀廷,郑晓丽. 用气相色谱法检测甲型肝炎减毒活疫苗中三氯甲烷含量[J]. 微生物学杂志, 2003, 23(6): 33-34 |
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| 作者姓名： | 沈红杰 辛暨华 全爱顺 姜崴 张耀廷 郑晓丽 |
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| 作者单位： | 长春生物制品研究所,吉林,长春,130062 |
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| 摘 要： | 用气相色谱法检测甲型肝炎减毒活疫苗中的三氯甲烷含量。以正丙醇作内标,用峰高代替峰面积,采用HP INNOW_(ax)毛细管柱,程序升温方法,准确定量甲型肝炎减毒活疫苗中的三氯甲烷含量。结果显示,色谱法优于限量法,能准确定量,快速灵敏。色谱法可用于实际检测分析。
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| 关 键 词： | 甲型肝炎减毒活疫苗 三氯甲烷 气相色谱 |
| 文章编号： | 1005-7021(2003)06-0033-02 |
| 修稿时间： | 2003-07-08 |
Determination of Trichloro Methane in Hepatitis Vaccine B by Gas Chromatography |
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| SHEN Hongjie,XIN Jihua,QUAN Aishun,JIANG Wei,ZHANG Yaoting,ZHENG Xiaoli. Determination of Trichloro Methane in Hepatitis Vaccine B by Gas Chromatography[J]. Journal of Microbiology, 2003, 23(6): 33-34 |
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| Authors: | SHEN Hongjie XIN Jihua QUAN Aishun JIANG Wei ZHANG Yaoting ZHENG Xiaoli |
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| Affiliation: | Rescarch Institute of Biology Product in Changchun JiLin 130062 |
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| Abstract: | To establish a gas chromatography method for determining the content of trichloro methane in Hepatitis Vaccine. Using peak to substitute acreage to calculate the content of trichloro methane in Hepatitis Vaccine beyond the membrane. HP INNOWax capillary column was used. Internal standard method was applied, and propyl alcohole was used as internal standard substance.This gas chromatography method is superior to the limitation method. This method is fit for practically detecting. |
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| Keywords: | Hepatitis Vaccine trichloro gas chromatography. |
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