| 嗜麦芽窄食单胞菌OUC_Est10全基因组测序及脂类水解酶多样性分析 |
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| 引用本文： | 董浩,芮俊鹏,孙建安,李香真,毛相朝.嗜麦芽窄食单胞菌OUC_Est10全基因组测序及脂类水解酶多样性分析[J].微生物学报,2017,57(11):1716-1721. |
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| 作者姓名： | 董浩 芮俊鹏 孙建安 李香真 毛相朝 |
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| 作者单位： | 中国海洋大学食品科学与工程学院, 山东 青岛 266003,中国科学院成都生物研究所, 环境与应用微生物重点实验室, 环境微生物四川省重点实验室, 四川 成都 610041,中国海洋大学食品科学与工程学院, 山东 青岛 266003,中国科学院成都生物研究所, 环境与应用微生物重点实验室, 环境微生物四川省重点实验室, 四川 成都 610041,中国海洋大学食品科学与工程学院, 山东 青岛 266003 |
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| 基金项目： | 国家自然科学基金（31501516）；山东省重大特殊科技项目（2016YYSP016）；青岛市应用基础研究项目（16-5-1-18-jch）；中央高校基金（201564018） |
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| 摘 要： | 目的]本研究的目的是研究嗜麦芽窄食单胞菌OUC_Est10中脂类水解酶的多样性。方法]使用离子交换层析、全基因组测序和异源表达三种方法研究嗜麦芽窄食单胞菌OUC_Est10中脂类水解酶的多样性。结果]离子交换层析结果显示嗜麦芽窄食单胞菌OUC_Est10可以分泌多种脂类水解酶。通过全基因组测序，我们给出了该菌的全基因组序列，该基因组大小为4668743 bp，GC含量为66.25%。通过详细的基因组序列分析，我们从该基因组中找到33个可能具有脂类水解酶活性的假定基因。通过异源表达OUC_Est10中的5个假定脂类水解酶基因，来研究其催化特性的多样性，结果显示这些脂类水解酶具有不同的催化特性。结论]我们证明了嗜麦芽窄食单胞菌OUC_Est10拥有多样的脂类水解酶，这暗示了它在不同领域中的应用潜力。
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| 关 键 词： | 嗜麦芽窄食单胞菌 分离 脂类水解酶 全基因组测序 催化特性 |
| 收稿时间： | 2017/5/3 0:00:00 |
| 修稿时间： | 2017/8/5 0:00:00 |
Complete genome sequencing and diversity analysis of lipolytic enzymes in Stenotrophomonas maltophilia OUC_Est10 |
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| Hao Dong,Junpeng Rui,Jianan Sun,Xiangzhen Li and Xiangzhao Mao.Complete genome sequencing and diversity analysis of lipolytic enzymes in Stenotrophomonas maltophilia OUC_Est10[J].Acta Microbiologica Sinica,2017,57(11):1716-1721. |
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| Authors: | Hao Dong Junpeng Rui Jianan Sun Xiangzhen Li and Xiangzhao Mao |
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| Institution: | College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong Province, China,Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan Province, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong Province, China,Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan Province, China and College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong Province, China |
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| Abstract: | Objective] The aim of this study was to study the diversity of lipolytic enzymes in Stenotrophomonas maltophilia OUC_Est10.Methods] Ion exchange chromatography, genome sequencing and heterologous expression were used to study the diversity of lipolytic enzymes in Stenotrophomonas maltophilia OUC_Est10.Results] Stenotrophomonas maltophilia OUC_Est10 could secret a wide range of lipolytic enzymes (lipases and esterases) as revealed by ion exchange chromatography. The complete genome is of 4668743 bp in length, with an average GC content of 66.25%. Genome annotation indicated the presence of 33 candidate genes whose products possess the predicted lipolytic enzyme activities. Analysis of catalytic features was carried out by expressing five putative lipolytic enzyme genes, and lipolytic enzymes in OUC_Est10 had different catalytic properties.Conclusion] We proved that Stenotrophomonas maltophilia OUC_Est10 was a good candidate to produce diverse lipolytic enzymes, with potential applications in various fields. |
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| Keywords: | Stenotrophomonas maltophilia separation lipolytic enzyme complete genome sequence catalytic properties |
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