| 植物基因替换编辑技术研究进展 |
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| 引用本文： | 王虹麟,张从省,刘昌林,谢传晓. 植物基因替换编辑技术研究进展[J]. 生物工程学报, 2017, 33(10): 1723-1732 |
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| 作者姓名： | 王虹麟 张从省 刘昌林 谢传晓 |
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| 作者单位： | 1中国农业科学院作物科学研究所，北京 100081;2安徽农业大学 生命科学学院，安徽 合肥 230036;3中国农业科学院作物科学研究所 作物分子育种国家工程实验室，北京 100081,1中国农业科学院作物科学研究所，北京 100081;2安徽农业大学 生命科学学院，安徽 合肥 230036;3中国农业科学院作物科学研究所 作物分子育种国家工程实验室，北京 100081,1中国农业科学院作物科学研究所，北京 100081;3中国农业科学院作物科学研究所 作物分子育种国家工程实验室，北京 100081,1中国农业科学院作物科学研究所，北京 100081;3中国农业科学院作物科学研究所 作物分子育种国家工程实验室，北京 100081 |
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| 基金项目： | 中国农业科学院基本科研业务费专项院级统筹项目 (No. Y2017XM03) 资助。 |
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| 摘 要： | 基因替换编辑是通过核酸酶介导的对目标基因进行定点敲入或替换的编辑技术,可以实现目的基因的定向修饰。本文从基因替换编辑技术的原理、实现方式与影响因素、应用及其前景等进行了总结与讨论,以期为通过定点基因替换或敲入技术开展高等植物基因功能鉴定与遗传改良研究提供参考。
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| 关 键 词： | 基因替换，定点敲入，基因功能鉴定，遗传改良 |
| 收稿时间： | 2017-05-10 |
Advances in targeted replacement genome editing in plants |
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| Honglin Wang,Congsheng Zhang,Changlin Liu and Chuanxiao Xie. Advances in targeted replacement genome editing in plants[J]. Chinese journal of biotechnology, 2017, 33(10): 1723-1732 |
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| Authors: | Honglin Wang Congsheng Zhang Changlin Liu Chuanxiao Xie |
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| Affiliation: | 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 School of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui, China; 3 National Engineering Laboratory of Crop Molecular Breeding Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China,1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 School of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui, China; 3 National Engineering Laboratory of Crop Molecular Breeding Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China,1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 National Engineering Laboratory of Crop Molecular Breeding Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China and 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 National Engineering Laboratory of Crop Molecular Breeding Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Targeted replacement genome editing refers to DNA modification and engineering technology that could induce and achieve mutations of targeted gene replacement or knockin at a target gene or DNA region. In this review, the principles, implementation methods, factors that influence efficiency and accuracy, and applications of gene replacement editing were summarized and discussed. It provides the reference for gene functional characterization and genetic improvements through gene replacement strategies in higher plant especially crops. |
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| Keywords: | targeted replacement targeted knock-in gene function identification crops genetic improvement |
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