| 细菌CRISPR-Cas系统的研究进展 |
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| 引用本文： | 胡丽,陈实. 细菌CRISPR-Cas系统的研究进展[J]. 微生物学报, 2017, 57(11): 1643-1652 |
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| 作者姓名： | 胡丽 陈实 |
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| 作者单位： | 武汉大学药学院, 湖北 武汉 430072,武汉大学药学院, 湖北 武汉 430072 |
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| 基金项目： | 国家自然科学基金（31670086） |
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| 摘 要： | 获得性免疫长期以来被视为真核生物所独有,而CRISPR-Cas系统的发现则打破了这一定论。它是广泛存在于细菌和古菌中的一种获得性免疫系统,通过捕获整合初次感染的外源核酸片段,在Cas蛋白与cr RNA(CRISPR RNAs)的共同作用下抵御相同核酸的再次入侵,以保护宿主免受侵扰。近些年,CRISPR-Cas系统得到广泛的关注和研究。本文主要从细菌微生物角度,对系统分类、作用机制及原核领域应用等取得重要突破的研究进行扼要阐述,为CRISPR-Cas系统的深入探究和应用拓展提供有价值的参考信息。
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| 关 键 词： | CRISPR-Cas系统 分类 作用机制 原核领域应用 |
| 收稿时间： | 2017-04-04 |
| 修稿时间： | 2017-06-01 |
Research progress of CRISPR-Cas system in bacteria |
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| Li Hu and Shi Chen. Research progress of CRISPR-Cas system in bacteria[J]. Acta microbiologica Sinica, 2017, 57(11): 1643-1652 |
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| Authors: | Li Hu and Shi Chen |
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| Affiliation: | School of Pharmaceutical Sciences, Wuhan University, Wuhan 430072, Hubei Province, China and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430072, Hubei Province, China |
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| Abstract: | The discovery of CRISPR-Cas system breaks certain theory which adaptive immunity had been long time considered as unique characteristics of eukaryotic organism. CRISPR-Cas, a new adaptive immunity system widespread in bacteria and archaea, protects the host from invasion of exogenous nucleic acids by capturing and using the Cas protein and crRNA to resisting it when invading again. In recent years, CRISPR-Cas system has aroused extensive attention and exploration. From microbial perspective, this article introduces a brief overview on classification, mechanism and application which have achieved significant breakthrough to provide valuable reference for further research on CRISPR-Cas system and its application. |
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| Keywords: | CRISPR-Cas system classification mechanism application in prokaryotic |
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