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A comparison of the thermostability of cellulases from various thermophilic fungi
Authors:G Wojtczak  C Breuil  J Yamada  J N Saddler
Institution:(1) Biotechnology & Chemistry Department, Forintek Canada Corp., 800 Montreal Road, K1G 3Z5 Ottawa, Ontario, Canada
Abstract:Summary The cellulase activities of six thermophilic fungi were compared. Although the thermophilic fungi grew at relatively high temperatures (>45°C) the optimum temperatures for assaying the various cellulase activities were only slightly higher than the optimum temperatures for the mesophilic fungi, Trichoderma harzianum. Over prolonged incubation (> 24 h) the thermophilic strains demonstrated a higher hydrolytic potential as a result of the greater thermostability of the cellulase components. Although the extracellular cellulase activities had similar pH and temperature optima, in some cases the thermostability of the extracellular components were considerably lower.
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