Effects of some drugs on the activity of glucose 6-phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro |
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Authors: | Ciftci Mehmet |
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Institution: | Department of Chemistry, Arts and Science Faculty, Ataturk University, Erzurum, Turkey. ciftcim@atauni.edu.tr |
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Abstract: | Inhibitory effects of some drugs on glucose 6-phosphate dehydrogenase from the erythrocytes of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) were investigated. The enzyme was purified 2488-fold in a yield of 76.8% using ammonium sulfate precipitation and 2',5'-ADP Sepharose 4B affinity gel at 4 degrees C. The drugs pental sodium, MgSO4, vancomycin, metamizol, marcaine, and prilocaine all exhibited inhibitory effects on the enzyme. While MgSO4 (K(i) = 12.119 mM), vancomycin (K(i) = 1.466 mM) and metamizol (K(i) = 0.392 mM) showed competitive inhibition, pental sodium (K(i) = 0.748 mM) and marcaine (K(i) = 0.0446 mM) displayed noncompetitive inhibition. |
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