家蚕中肠组织抗核型多角体病毒病的相关蛋白分析

家蚕中肠上皮是病毒经口侵入遇到的第一个组织。昆虫幼虫抵御杆状病毒的感染，可通过选择性的使感染的中肠上皮细胞发生调亡并在释放病毒粒子进入血淋巴之前使感染的细胞从中肠脱落。为研究家蚕抗核型多角体病毒(Bombyx mori nucleopolyhedrovirus, BmNPV)病的机制，通过对BmNPV高度抗性和高度敏感性的家蚕品系杂交和回交构建了近等基因系。本文对家蚕高抗，敏感及近等基因系5龄起蚕中肠组织的蛋白质表达谱进行了二维电泳 (two-dimensional gel electrophoresis，2-DE) 分析，并利用基质辅助激光解吸电离飞行时间 (matrix-assisted laser desorption/ionization-time of flight, MALDI-TOF) 质谱对差异蛋白进行鉴定。结果发现了5个差异表达的蛋白。推测这些蛋白可能与家蚕中肠对BmNPV的抗性或感性有关。

Proteome analysis of silkworm(Bombyx mori)midgut proteins related to BmNPV infection resistance or susceptibility

The midgut epithelium is the first tissue of silkworm (Bombyx mori) which the virus confronts; larvae resist baculovirus infection by selective apoptosis of infected midgut epithelial cells and by sloughing off infected cells from the midgut before they release virions into the hemocoel. To research the mechanism of nucleopolyhedrovirus resistance of silkworm midgut tissue, we constructed a near-isogenic silkworm line with BmNPV resistance through highly resistant and highly susceptible parent strains. In this study, proteome maps and differences of protein patterns of the midgut tissues of the highly resistant, highly susceptible and near-isogenic silkworm strains were investigated by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. We compared the proteomic expression of the midgut in these three silkworm strains, and identified five differently expressed proteins. It is inferred that these proteins may be involved in the resistance or susceptibility of BmNPV.