The effect of oxygen transfer on the activity of encapsulated whole cell β-galactosidase |
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Authors: | J K Park G S Jeong H N Chang |
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Institution: | Dept. of Chem. Eng., Kyungpook National University, 702-701 Taegu, Korea, KR BioProcess Engineering Research Center and Dept. of Chemical Engineering, KAIST, Daedok Science Town, 305-701 Taejon, Korea, KR
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Abstract: | The effect of oxygen transfer on the production of immobilized whole cell β-galactosidase has been evaluated. The encapsulated whole cell β-galactosidase was prepared by combining cell encapsulation and culture into one-step. Escherichia coli was encapsulated and cultured in the growth and production media to accumulate β-galactosidase in itself. Sunflower seed oil was coimmobilized to increase the oxygen transfer rate through the capsule membrane. The oxygen transfer rate increased 63 percent and the activity of β-galactosidase increased by 10 percent. The activity of encapsulated β-galactosidase obtained in the concentric air lift reactor was 86 percent higher than that in the shaking incubator. In the concentric air lift reactor, the accumulation of encapsulated whole cell β-galactosidase was primarily dependent on the capsule velocity. While the accumulation of specific β-galactosidase in the capsule increased with volumetric oxygen transfer coefficient, the cell biomass accumulated in the capsule decreased. |
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