Assessment of mechanical properties of adherent living cells by bead micromanipulation: comparison of magnetic twisting cytometry vs optical tweezers |
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Authors: | Laurent Valérie M Hénon Sylvie Planus Emmanuelle Fodil Redouane Balland Martial Isabey Daniel Gallet François |
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Affiliation: | INSERM Unité 492, Physiopathologie et Thérapeutique Respiratoires, Faculté de Médecine, Université Paris XII, Créteil, France. v.laurent@creitel.inserm.fr |
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Abstract: | We compare the measurements of viscoelastic properties of adherent alveolar epithelial cells by two micromanipulation techniques: (i) magnetic twisting cytometry and (ii) optical tweezers, using microbeads of same size and similarly attached to F-actin. The values of equivalent Young modulus E, derived from linear viscoelasticity theory, become consistent when the degree of bead immersion in the cell is taken into account. E-values are smaller in (i) than in (ii): approximately 34-58 Pa vs approximately 29-258 Pa, probably because higher stress in (i) reinforces nonlinearity and cellular plasticity. Otherwise, similar relaxation time constants, around 2 s, suggest similar dissipative mechanisms. |
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