Mitochondrial cox1 and plastid rbcL genes of Gracilaria vermiculophylla (Gracilariaceae,Rhodophyta) |
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Authors: | Eun Chan Yang Myung Sook Kim Paul John L. Geraldino Dinabandhu Sahoo Jong-Ahm Shin Sung Min Boo |
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Affiliation: | (1) Department of Biology, Chungnam National University, Gungdong 220, Daejeon, 305-764, South Korea;(2) Research Institute of Basic Sciences, Pusan National University, Pusan, 609-735, South Korea;(3) Marine Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi, 110007, India;(4) Fisheries and Ocean Science, Chonnam National University, Yeosu, 550-749, South Korea |
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Abstract: | The mitochondrial cytochrome c oxidase subunit I gene sequence was recently developed for DNA barcoding of red algal species. We determined the 1245 base pairs of the gene from 27 taxa of an agar-producing species, Gracilaria vermiculophylla, and putative relatives and compared the results with rbcL data from the same species. A total of 392 positions (31.5%) were variable, 282 positions (22.6%) were parsimoniously informative, and average sequence divergence was 13% in an ingroup. Within G. vermiculophylla, pairwise divergence of the gene was variable up to 11 bp (0.9%). Seven recognized haplotypes of cox1 tended to be geographically related. In the aligned 1386 bp of rbcL, three haplotypes were recognized. These results suggest that cox1 is a valuable molecular marker within species and will be very useful in haplotype analyses. |
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Keywords: | DNA barcoding Gracilariales Haplotype Molecular marker Phylogeny |
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