Removal of carboxymethyl cellulase activity from the culture filtrate of Termitomyces clypeatus producing xylanase |
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Authors: | A.K. Ghosh M.L. Jana A.K. Naskar R. Chatterjee S. Sengupta |
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Affiliation: | (1) Department of Applied Biochemistry, Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata, 700 032, India |
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Abstract: | Termitomyces clypeatus produced 450 IU xylanase ml–1 in a medium containing starch-free wheat bran powder as the carbon source. Carboxymethyl cellulase (CMCase) activity in the culture filtrate was removed by keeping the filtrate at pH 10 for 60 min followed by a change to pH 6. Treatment of Kraft pulp (bamboo) with the filtrate at pH 7 decreased the kappa number from 10.5 to 5 with release of reducing groups equivalent to 0.15 mg glucose g–1 pulp. |
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Keywords: | biobleaching cellulase free xylanase Termitomyces clypeatus xylanase |
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