Use of immobilized cells of the strainCorynebacterium sp. for 4-nitrophenol degradation |
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Authors: | L Kotoučková J Vavřík M Němec J Plocek Z Zdráhal |
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Institution: | (1) Department of Microbiology, Faculty of Science, Masaryk University, 602 00 Brno, Czech Republic;(2) Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, 611 42 Brno, Czech Republic |
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Abstract: | 4-Nitrophenol degrading bacterial strainCorynebacterium sp. 8/3 was isolated from chemically polluted soil. The product of cometabolic transformation of 4-nitrophenol was identified
as 4-nitrocatechol., Effect of immobilization (encapsulation in calcium alginate) ofCorynebacterium sp. cells on the process of 4-nitrophenol transformation was investigated. 4-Nitrophenol was converted by encapsulated cells
and encapsulation had a protective effect, on 4-nitrophenol degrading bacteria in repeated cycles of incubation. Transformation
of 4-nitrophenol to 4-nitrocatechol by encapsulated cells was influenced by pH of medium but was not influenced by concentration
of alginate and CaCl2. The count of viable cells in alginate beads declined approximately by one order of magnitude after 10 d of incubation.
Presented at the 4th Mini-Symposium on Biosorption and Microbial Degradation, Prague, Czech Republic, November 26–29, 1996. |
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