| 四苯基卟啉氯化锰与ctDNA的相互作用 |
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| 引用本文： | 朱卫华,周燕,练翠翠,李敏智,陈秋云,欧忠平,许化溪.四苯基卟啉氯化锰与ctDNA的相互作用[J].生物技术,2011,21(3). |
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| 作者姓名： | 朱卫华 周燕 练翠翠 李敏智 陈秋云 欧忠平 许化溪 |
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| 作者单位： | 1. 江苏大学化学化工学院,江苏镇江,212013
2. 江苏大学基础医学与医学技术学院,江苏镇江,212013 |
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| 基金项目： | 国家自然科学基金项目(调节线粒体功能的抗肿瘤活性锰配合物的合成及构效关系研究,20971059多功能咔咯配合物的合成及催化性能和构效关系研究,21071067)资助 |
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| 摘 要： | 目的:探究非水溶性的四苯基卟啉氯化锰(TPP)MnCl]与小牛胸腺DNA(ctDNA)的相互作用方式及机理.方法:借助紫外-可见光谱、荧光光谱和电化学等手段研究了(TPP)MnCl与ctDNA相互作用的过程.结果:两者作用后,(TPP)MnCl的紫外-可见光谱在Soret带减色30%;EB-DNA体系的荧光猝灭;(TPP)MnCl的循环伏安也明显变化.实验测得(TPP)MnCl与ctDNA的表观结合常数为9.3×105L·moL-1.结论:在实验条件下,(TPP)MnCl与ctDNA以静电结合方式相互作用.
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| 关 键 词： | 四苯基卟啉氯化锰 小牛胸腺DNA 相互作用 作用机理 结合常数 |
Interaction of 5,10,15,20-Tetraphenylporphine Manganese Chloride with ctDNA |
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| ZHU Wei-hua,ZHOU Yan,LIAN Cui-cui,LI Min-zhi,CHEN Qiu-yun,OU Zhong-ping,XU Hua-xi.Interaction of 5,10,15,20-Tetraphenylporphine Manganese Chloride with ctDNA[J].Biotechnology,2011,21(3). |
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| Authors: | ZHU Wei-hua ZHOU Yan LIAN Cui-cui LI Min-zhi CHEN Qiu-yun OU Zhong-ping XU Hua-xi |
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| Institution: | ZHU Wei-hua1,ZHOU Yan1,LIAN Cui-cui1,LI Min-zhi1,CHEN Qiu-yun1,OU Zhong-ping1,XU Hua-xi2*(1.School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,China,2.School of Medical Science and Laboratory Medicine,China) |
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| Abstract: | Objective:To study the method and mechanism for the interaction of the water-insoluble(TPP)MnCl with calf thymus DNA(ctDNA).Method:Using UV-visible spectroscopy,fluorescence spectroscopy and cyclic voltammetry to trace the process of the interaction of(TPP)MnCl with ctDNA.Result: With the interaction of(TPP)MnCl and ctDNA,30% hypochromic effect was observed in the Soret band of UV-vis spectrum for(TPP)MnCl,the fluorescence of EB-DNA was quenched and the cyclic voltammogram of(TPP)MnCl was changed greatly.Th... |
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| Keywords: | (TPP)MnCl ctDNA interaction mechanism binding constant |
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