Self-renewal and differentiation capabilities are variable between human embryonic stem cell lines I3, I6 and BG01V |
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| Authors: | Tahereh Tavakoli Xiangru Xu Eric Derby Yevgeniya Serebryakova Yvonne Reid Mahendra S Rao Mark P Mattson and Wu Ma |
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| Institution: | (1) Stem Cell Center, Developmental Biology, American Type Culture Collection (ATCC), Manassas, VA, USA;(2) Laboratory of Neurosciences, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA;(3) Stem Cells and Regenerative Medicine (Research), Invitrogen, Carlsbad, CA, USA;(4) iZumi Bio Inc., 951 Gateway Boulevard, South San Francisco, CA 94080, USA |
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| Abstract: | Background A unique and essential property of embryonic stem cells is the ability to self-renew and differentiate into multiple cell
lineages. However, the possible differences in proliferation and differentiation capabilities among independently-derived
human embryonic stem cells (hESCs) are not well known because of insufficient characterization. To address this question,
a side-by-side comparison of 1) the ability to maintain an undifferentiated state and to self-renew under standard conditions;
2) the ability to spontaneously differentiate into three primary embryonic germ lineages in differentiating embryoid bodies;
and 3) the responses to directed neural differentiation was made between three NIH registered hES cell lines I3 (TE03), I6
(TE06) and BG01V. Lines I3 and I6 possess normal XX and a normal XY karyotype while BG01V is a variant cell line with an abnormal
karyotype derived from the karyotypically normal cell line BG01. |
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