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| 生防工程菌株BS-17A稳定性与抑菌活性初步研究 | |
| 引用本文： | 于德水,高娃,沙长青,张淑梅,高继国.生防工程菌株BS-17A稳定性与抑菌活性初步研究[J].生物技术,2011,21(3). |
| 作者姓名： | 于德水 高娃 沙长青 张淑梅 高继国 |
| 作者单位： | 1. 黑龙江省科学院高技术研究院,黑龙江哈尔滨,150030 2. 黑龙江省科学院微生物研究所,黑龙江哈尔滨,150010 3. 东北农业大学生命科学学院,黑龙江哈尔滨,150030 |
| 摘 要： | 目的:BS-17是内生细菌,对番茄灰霉病菌、叶霉病菌和枯萎病菌具有显著抑菌活性,为了跟踪研究野生型菌株BS-17在番茄根围和叶围的定殖情况,构建了1株带有黄绿荧光蛋白基因标记的生防菌株BS-17A.方法:采用NYD连续培养的方法和平皿抑菌试验的方法对工程菌株的遗传稳定性和抑菌活性进行了初步研究.结果:该工程菌在无选择压力培养基中连续培养50h,质粒遗传稳定性为94%,对番茄灰霉病菌Botrytis cinerea、叶霉病菌Cladosporiumfulvum和枯萎病菌Fusarium oxysporum的抑制作用与野生菌无显著差异,平皿抑菌率分别为85.5%、86.5%和89.8%.结论:该工程菌具有较强的遗传稳定性和抑菌活性. |
| 关 键 词： | 工程菌株BS-17A 遗传稳定性 抑菌活性 |
Preliminary Studyies on Stability and Antifungal Activity of Biocontrol Engineered Strains BS-17A |
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| YU De-shui,GAO Wa,SHA Chang-qing,ZHANG Shu-mei,GAO Ji-guo.Preliminary Studyies on Stability and Antifungal Activity of Biocontrol Engineered Strains BS-17A[J].Biotechnology,2011,21(3). | |
| Authors: | YU De-shui GAO Wa SHA Chang-qing ZHANG Shu-mei GAO Ji-guo |
| Institution: | YU De-shui2,GAO Wa2,SHA Chang-qing3,ZHANG Shu-mei2,GAO Ji-guo1* (1.College of Life Science,Northeast of Agricultural University,Harbin 150030,2.Institute of High Technology,Heilongjiang Academy of Sciences,3.Institute of Microbiology,Harbin 150010,China) |
| Abstract: | Objective:Bacillus subtilis BS-17 is an endophytic bacteria.It strongly inhibits the growth of Botrytis cinerea,Cladosporium fulvum and Fusarium oxysporum,which cause tomato fungal diseases.To investigate the colonization of the bacterium on the tomato leaves and roots,the biocontrol bacterium Bacillus subtilis BS-17A had been labeled with yellow green fluorescent protein gene.Method:The plasmid stability and antifungal activity of the biocontrol engineered Bacillus subtilis BS-17A had been studied with the... |
| Keywords: | biocontrol engineered strains BS-17A genetic stability inhibition activities |
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