| 蓝色犁头霉深层发酵产壳低聚糖生长动力学研究 |
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| 引用本文： | 王伟平,陈维,韩凤云,杜予民.蓝色犁头霉深层发酵产壳低聚糖生长动力学研究[J].生物技术,2011,21(3). |
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| 作者姓名： | 王伟平 陈维 韩凤云 杜予民 |
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| 作者单位： | 1. 湖北工业大学生物工程学院,湖北武汉,430068
2. 武汉大学资源与环境科学学院,湖北武汉,430072 |
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| 摘 要： | 目的:研究蓝色犁头霉产壳低聚糖分批发酵动力学的模型.方法:在10L发酵罐中,将蓝色犁头霉分批发酵培养,对蓝色犁头霉菌丝体生长、产物形成和基质消耗的实验数据进行分析,根据Logistic和Luedeking-Piret方程分别建立蓝色犁头霉发酵过程菌体生长、壳低聚糖生成和基质消耗的动力学数学模型,并利用1stOpt软件对模型参数进行非线性曲线拟合.结果:研究得到了菌体生长、产物合成及基质消耗的动力学模型及参数,模型的拟合度分别为0.994、0.986、0.992.结论:研究表明蓝色犁头霉多糖合成和菌体生长呈生长偶联型,模型计算值与实验值有良好的拟合性,模型准确度较高.
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| 关 键 词： | 蓝色犁头霉 壳低聚糖 发酵动力学模型 |
Kinetic Study of the Low-molecular-chitosan Formation by Absidia coerulea |
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| WANG Wei-ping,CHEN Wei,HAN Feng-yun,DU Yu-min.Kinetic Study of the Low-molecular-chitosan Formation by Absidia coerulea[J].Biotechnology,2011,21(3). |
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| Authors: | WANG Wei-ping CHEN Wei HAN Feng-yun DU Yu-min |
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| Institution: | WANG Wei-ping1,CHEN Wei1,HAN Feng-yun1,DU Yu-min2(1.College of Bioengineering,Hubei University of Technology,Wuhan 430068,China,2.College of Resource and Environmental Science,Wuhan University,Wuhan 430072,China) |
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| Abstract: | Objective:To evaluate the kinetic models of Absidia coerulea producing low molecular chitosan(LMC) in Batch fermentation.Method:Absidia coerulea was cultured in 10L fermentor,and then the data of cell growth,product formation and substrate consumption were analyzed.Based on the Logistic and Luedeking-Piret equations,the mathematic models of cell growth,LMC production and substrate consumption were developed to describe the Absidia coerulea fermentation process.The parameters of the models were fitted by 1st... |
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| Keywords: | 1stOpt |
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