Ion Content of the Halotolerant Alga Dunaliella salina |
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Authors: | Karni, Leah Avron, Mordhay |
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Affiliation: | Biochemistry Department, Weizmann Institute of Science Rehovot 76100, Israel |
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Abstract: | The intracellular concentration of the major ions in Dunaliellasalina cells were determined, following the removal of extracellularions by ion-exchange minicolumns. Log phase cells, grown inmedia containing 14 molar NaCl, contained 3050mM chloride and 200350 mM magnesium (5 mM in medium).Phosphorus, which is present intracellularly mostly as polyphosphate,was present in amounts of 60100 fmoles per cell, equivalentto a concentration of 6001,000 mM (0.2 mM in medium).Previous data indicated that such cells contained 2040mM Na+, 150300mM K+, 20mM SO24, and very low concentrationsof Ca2+ and charged nitrogenous compounds. Mg2+ and K+ seemto serve as the major counter ions for the intracellular negativecharge present in the massively accumulated polyphosphates.The former accounts for about 2/3 of the required positive charge.This is supported by the observation that limitation in thephosphate or K+ supply in the medium lead to a parallel decreasein the accumulation of intracellular phosphorus, Mg2+ or K+. 1Present address: Department of Vegetables, The Volcani Center,Bet-Dagan 50250, Israel. (Received June 13, 1988; Accepted August 25, 1988) |
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