Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria: role of D-alanine racemase |
| |
| Authors: | Halouska Steven Chacon Ofelia Fenton Robert J Zinniel Denise K Barletta Raul G Powers Robert |
| |
| Affiliation: | Department of Chemistry, University of Nebraska Lincoln, Lincoln, Nebraska 68522, USA. |
| |
| Abstract: | D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
