Mycoplasma in african green monkey kidney cell cultures: Biochemical detection and effects in virus-infected cells |
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Authors: | F Van Roy Professor W Fiers |
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Institution: | (1) Laboratory of Molecular Biology, University of Ghent, Ledeganckstraat 35, B-9000 Gent, Belgium |
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Abstract: | Summary Among a number of techniques for the detection of mycoplasmal contamination in African green monkey kidney (AGMK) cell lines,
the assay of uridine phosphorylase activity is unsuitable because of the presence of high levels of endogenous enzymatic activity.
A thymidine phosphorylase test, however, based on the chromatographic analysis of radiolabeled thymidine breakdown, turned
out to be a simple and sensitive mycoplasma detection method.
We found, using the latter technique, that 0.22-μm-filtered virus inocula could still transfer mycoplasma unless treated with
diethyl ether. The effect of mycoplasmal contamination on the synthesis of simian virus 40 and adenovirus in AGMK cells was
negligible under the conditions used (no depletion of arginine). Incorporation of radioactive thymidine in viral macromolecules,
however, was inhibited severely by the presence of mycoplasma.
This investigation was supported by a grant from theFonds voor Geneeskundig Wetenschappelijk Onderzoek (No. 20.298). F.V. R. is an Aspirant of the BelgianNationaal Fonds voor Wetenschappelijk Onderzoek. |
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Keywords: | mycoplasma AGMK cells biochemical detection thymidine phosphorylase virus infection |
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