Photoregulation of Protochlorophyllide Oxidoreductase and Rubisco Large Subunit Accumulation in Phytochrome A-Deficient Transgenic Tobacco Plants |
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| Authors: | Antipova T V Gapeeva T A Volotovsky I D |
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| Institution: | (1) Institute of Photobiology, Belarussian Academy of Sciences, Akademicheskaya ul. 27, Minsk, 220072, Belarus; fax |
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| Abstract: | Some morphogenetic responses, induced by far red (FR) light in tobacco plants (Nicotiana tabacum L.), were studied. The inhibitory effect of FR irradiation on chlorophyll synthesis in transgenic plants with reduced phytochrome A content was almost absent. Phytochrome A-mediated repression of the por gene was demonstrated with the use of polyclonal antiserum against protochlorophyllide oxidoreductase. Continuous FR light induced the accumulation of Rubisco large subunits in wild-type but not in transgenic tobacco plants. Our data confirm the suggestion that phytochrome A mediates photoregulation of the synthesis of these proteins. |
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| Keywords: | Nicotiana tabacum transgenic plants asRNA phytochrome A FR-block of greening NADPH:protohlorophyllide oxidoreductase Rubisco |
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