Virus-induced silencing of WIPK and SIPK genes reduces resistance to a bacterial pathogen,but has no effect on the INF1-induced hypersensitive response (HR) in Nicotiana benthamiana |
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| Authors: | Sharma P C Ito A Shimizu T Terauchi R Kamoun S Saitoh H |
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| Affiliation: | (1) Department of Agricultural Botany, Ch. Charan Singh University, 250004 Meerut, India;(2) Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, Japan;(3) Department of Plant Pathology, Ohio Agricultural Research and Development Center, Ohio State University, Wooster, OH 44691, USA;(4) Japan Society for the Promotion of Science, 5-3-1 Koujimachi, Chiyoda-ku, Tokyo 102-8471, Japan;(5) Present address: School of Biotechnology, Guru Gobind Singh Indraprastha University, 110006 Delhi, India;(6) Present address: Department of Plant Microbe Interactions, Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany |
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| Abstract: | Activation of two mitogen-activated protein kinases (MAPKs), wound-induced protein kinase (WIPK) and salicylic acid-induced protein kinase (SIPK), is one of the earliest responses that occur in tobacco plants that have been wounded, treated with pathogen-derived elicitors or challenged with avirulent pathogens. We isolated cDNAs for these MAPKs ( NbWIPK and NbSIPK) from Nicotiana benthamiana. The function of NbWIPK and NbSIPK in mediating the hypersensitive response (HR) triggered by infiltration with INF1 protein (the major elicitin secreted by Phytophthora infestans), and the defense response to an incompatible bacterial pathogen ( Pseudomonas cichorii), was investigated by employing virus-induced gene silencing (VIGS) to inhibit expression of the WIPK and SIPK genes in N. benthamiana. Silencing of WIPK or SIPK, or both genes simultaneously, resulted in reduced resistance to P. cichorii, but no change was observed in the timing or extent of HR development after treatment with INF1.Communicated by R. G. Herrmann |
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| Keywords: | Nicotiana benthamiana WIPK/SIPK INF1 Pseudomonas cichorii Virus-induced gene silencing |
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