微囊藻毒素对斑马鱼重要细胞因子表达的影响

微囊藻毒素是一种肝毒素,它攻击的靶位是肝细胞,能导致鱼的肝脏发生病理变化。该研究以斑马鱼为研究对象,采用RT-PCR方法研究了微囊藻毒素对斑马鱼白细胞介素1β(Interleukin 1β,IL-1β),白细胞介素8(Interleukin 8,IL-8),白细胞介素10(Interleukin 10,IL-10),干扰素(Interferon,IFN),肿瘤坏死因子(Tumor Necrosis Factor,TNF),CXC,CXCa等几个重要细胞因子表达的影响。实验表明,25d时,仅毒素组的斑马鱼肝中有IFN和TNF表达。35d时,毒素组斑马鱼鳃中表达了IL-10,肝中表达了CXCa;毒素组和对照组的斑马鱼肝中都表达了IL-1β,TNF,IL-10,CXC,IFN,且其IL-1β,TNF,IL-10的条带亮于对照组。45d时,IFN仅在毒素组鱼鳃和肝中检测到表达;CXC仅在毒素组的鳃中检测到表达;IL-10在对照组的鳃、肠、肾和肌肉中有表达,在毒素组的肝、卵、肾、肌肉中有表达;IL-1β在对照组和毒素组的肝、卵、肾和肌肉中均有表达,且其条带亮于对照组。结果表明:毒素组斑马鱼的肝和鳃组织发生了炎症反应。这为进一步研究微囊藻毒素对鱼类免疫系统的毒害作用奠定了基础。

Influences of Microcystins on several significant cytokines of zebra fish, Danio rerio

Microcystins are hepatotoxic,causing necrosis and pooling of blood in the liver of fish.In this paper,RT-PCR was used to study the effects caused by microcystins on several important cytokines of zebra fish such as interleukin-1β(IL-1β),IL-8,IL-10,interferon(IFN),tumor necrosis factor(TNF),CXC and CXCa.Sixty zebra fish were divided into experimental group and the control,thirty in each group.The experimental group was cultivated outdoor exposing to microcystins,and the control cultivated indoor with water replacement daily.The results showed that:1) after 25 days,IFN and TNF in the liver of experimental group could be detected; 2) after 35 days,IL-10 in the gill of experimental group,CXCa in the liver of experimental group,IL-1β,TNF,IL-10,CXC and IFN in the liver of both groups were observed,and the expressions of IL-1β,TNF,IL-10 in experimental fish were higher than those of the control; 3) after 45 days,IFN in the gill and liver of experimental group,CXC in the gill of experimental group,IL-10 in gill,intestines,kidney and muscle of control group,IL-10 in liver,egg,kidney and muscle of experimental group,IL-1β in liver,egg,kidney and muscle of both groups were expressed,but expressions of IL-1β in experimental group were higher than those of the controls.In conclusion,these results suggested that the inflammation occurred in liver and gill of experimental group.