Sulbutiamine Counteracts Trophic Factor Deprivation Induced Apoptotic Cell Death in Transformed Retinal Ganglion Cells |
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| Authors: | Kui Dong Kang Aman Shah Abdul Majid Kyung-A Kim Kyungsu Kang Hong Ryul Ahn Chu Won Nho Sang Hoon Jung |
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| Institution: | (1) Department of Ophthalmology, Oxford Eye Hospital, University of Oxford, Headington, Oxford, UK;(2) Department of Ophthalmology, The Catholic University of Korea, Seoul, Korea;(3) Advanced Medical and Dental Institute, Universiti Sains Malaysia, Penang, Malaysia;(4) Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Daejeon-dong, Gangneung, 210-340, Korea; |
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| Abstract: | Sulbutiamine is a highly lipid soluble synthetic analogue of vitamin B1 and is used clinically for the treatment of asthenia. The aim of our study was to demonstrate whether sulbutiamine is able
to attenuate trophic factor deprivation induced cell death to transformed retinal ganglion cells (RGC-5). Cells were subjected
to serum deprivation for defined periods and sulbutiamine at different concentrations was added to the cultures. Various procedures
(e.g. cell viability assays, apoptosis assay, reactive oxygen species analysis, Western blot analysis, flow cytometric analysis,
glutathione (GSH) and glutathione-S-transferase (GST) measurement) were used to demonstrate the effect of sulbutiamine. Sulbutiamine
dose-dependently attenuated apoptotic cell death induced by serum deprivation and stimulated GSH and GST activity. Moreover,
sulbutiamine decreased the expression of cleaved caspase-3 and AIF. This study demonstrates for the first time that sulbutiamine
is able to attenuate trophic factor deprivation induced apoptotic cell death in neuronal cells in culture. |
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