Effect of PARP-1 deficiency on DNA damage and repair in human bronchial epithelial cells exposed to Benzo(a)pyrene |
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Authors: | Gong-hua Tao Lin-qing Yang Chun-mei Gong Hai-yan Huang Jian-dong Liu Jian-jun Liu Jian-hui Yuan Wen Chen Zhi-xiong Zhuang |
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Institution: | (1) Department of Preventive Medicine, School of Public Health, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, Guangdong, People’s Republic of China;(2) Shenzhen Center for Disease Control and Prevention, 21 Tianbei 1st Road, Shenzhen, 518020, Guangdong, People’s Republic of China; |
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Abstract: | Benzoa]pyrene is a ubiquitously distributed environmental pollutant known to cause DNA damage, whereas PARP-1 is a nuclear
enzyme that is activated by damaged DNA and plays an important role in base excision repair and genomic stability. Here, 16HBE
and its PAPR1-deficient cells were exposed to BaP, and the DNA damage level and repair ability of both cell lines were measured
by alkaline comet assay. The results showed that cell viability of both cell lines decreased in a dose-dependent manner when
exposed to BaP, but there was no significant difference between two cell lines. Comet assay showed that BaP caused DNA damage
in both cell lines at an obvious dose- and time-dependent manner. Compare with 16HBE, the PARP1-deficient cells were more
sensitive to the damage caused by BaP. The results of DNA repair experiment showed that both cell lines can recover from the
damage in a time-dependent pattern. The relative repair percentage of PARP1-deficient cells were generally lower than that
of 16HBE at all exposed concentrations at the early stage of repair, but tended to be closer between two cell lines at the
later period. According to results, we came to the conclusion that PARP1-deficient cells were more sensitive to BaP in contrast
to normal 16HBE; DNA repair capacity in PARP1-deficient cells decreased significantly at the early stage of repair, but increased
to the equivalent level of normal 16HBE in the later period. PARP-1 plays an important role in early repair of DNA damage
caused by BaP in 16HBE notwithstanding the main repair work is taken by NER pathway. |
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