Determination of releasing hormones in subcellular fractions from porcine hypothalamic tissue |
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| Authors: | K N Johansson B L Currie K Folkers |
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| Institution: | Institute for Biomedical Research The University of Texas at Austin Austin, Texas 78712 USA;Tulane University School of Medicine New Orleans, Louisiana 70112 USA |
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| Abstract: | In a study of possible biosynthesis of hypothalamic hormones in mitochondria, the activities of subcellular fractions from gradient Ficoll fractionation were assayed. Mitochondrial fractions identified by qualitative assays for oxygen uptake and phosphorylation, showed both respiratory activity and release of the luteinizing hormone. Purification of solvent extracts of mitochondrial fractions by Bio-Gel P-2 and Sephadex G-25 yielded fractions which released the luteinizing and follicle stimulating hormones and somatotropin. Labeled pGlu-His-Pro-NH2 and the release of thyrotropin served as a control. |
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