| 小麦—黑麦异代换及小麦种内易位系的分子细胞遗传学检测 |
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| 引用本文： | 郭长虹,石锐,王同昌,张艳,张百臣,唐力平,李集临.小麦—黑麦异代换及小麦种内易位系的分子细胞遗传学检测[J].西北植物学报,2001,21(3):413-418,T001. |
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| 作者姓名： | 郭长虹 石锐 王同昌 张艳 张百臣 唐力平 李集临 |
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| 作者单位： | 哈尔滨师范大学生物系 |
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| 基金项目： | 国家自然科学基金资助项目(3860514)"诱发染色体易位导入近缘种基因的研究"的延续。 |
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| 摘 要： | 研究应用基因组原位杂交、染色体C-分带和RAPD技术,对八倍体小黑麦×普通小麦杂种F2经电离辐射处理后的高代材料98-60进行了检测。基因组原位杂交结果表明,该材料为小麦-黑麦异代换系。进一步通过C-分带分析表明,该品系为5R代换系,并且还包含有5AS/6AS小麦种内的染色体易位。通过RAPD分析,在该品系中找到了来源于八倍体小黑麦亲本"新麦73"的黑麦染色体特异扩增产物OPA-01350和与两个亲本不同的特异重组产物OPF-14800、OPF-14920,进一步验证了基因组原位杂交和C-分带的鉴定结果。
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| 关 键 词： | 小麦 黑麦 原位杂交 C－分带 RAPD 异代换系 易位系 细胞遗传 |
| 文章编号： | 1000-4025-(2001)03-0413-06 |
Molecular cytogenetics detection of wheat-rye substitution and wheat intra-specific translocation line |
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| GUO Chang hong,SHI Rui,WANG Tong chang,ZHANG Yan,ZHANG Bai chen,TANG Li ping,LI Ji lin.Molecular cytogenetics detection of wheat-rye substitution and wheat intra-specific translocation line[J].Acta Botanica Boreali-Occidentalia Sinica,2001,21(3):413-418,T001. |
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| Authors: | GUO Chang hong SHI Rui WANG Tong chang ZHANG Yan ZHANG Bai chen TANG Li ping LI Ji lin |
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| Institution: | GUO Chang hong,SHI Rui,WANG Tong chang,ZHANG Yan,ZHANG Bai chen,TANG Li ping,LI Ji lin * |
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| Abstract: | GISH,chromosome C-banding and RAPD techniques were used in the detection of rye chromatin in high generation offspring (98-60) of radiation treated F2 of crossing between octoploid triticale and common wheat．According to the result of GISH,these offspring are substitution lines,further analysis by C-banding reveal the existence of 5R chromosome and a translocation of 5AS/6AS．These were also confirmed by RAPD that a amplified fragment,OPA-01350,derives from octoploid triticale line "Xinmai 73" and two amplified recombination products,OPF-14800 and OPF-14920,are different from genomic DNA of two parents. |
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| Keywords: | wheat rye GISH C-banding RAPD substitution translocation |
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