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A rapid screening and production method using a novel mammalian cell display to isolate human monoclonal antibodies
Authors:Kosuke Tomimatsu  Shin-ei Matsumoto  Hayato Tanaka  Makiko Yamashita  Hidekazu Nakanishi  Kiichiro Teruya  Saiko Kazuno  Tomoya Kinjo  Takeki Hamasaki  Ken-ichi Kusumoto  Shigeru Kabayama  Yoshinori Katakura  Sanetaka Shirahata
Affiliation:1. Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;2. Japan Society for the Promotion of Science, 8 Ichibancho, Chiyoda-ku, Tokyo 102-8472, Japan;3. Department of Diagnosis, Prevention and Treatment of Dementia, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan;4. Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;5. Biomedical Research Center, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan;6. Biotechnology and Food Research Institute Fukuoka Industrial Technology Center, 1465-5 Aikawa-cho, Kurume, Fukuoka 839-0861, Japan;g Nihon Trim Co., Ltd., 1-8-34 Oyodonaka, Kita-ku, Osaka 531-0076, Japan
Abstract:
Keywords:Human monoclonal antibody   Mammalian cell display   In vitro maturation   CHO cells   Cre recombinase   Human high affinity IgE receptor
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