Protein phosphatase 2A family members (PP2A and PP6) associate with U1 snRNP and the spliceosome during pre-mRNA splicing |
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| Authors: | Malek Kamoun Mohammed Filali Michael V. Murray Sita Awasthi Brian E. Wadzinski |
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| Affiliation: | 1. Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA;2. Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY, USA;3. Diosynth Biotechnology, 3000 Weston Parkway, Cary, NC, USA;4. Department of Medicine, Division of Infectious Diseases, University of Pennsylvania School of Medicine, Philadelphia, PA, USA;5. Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA |
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| Abstract: | Protein phosphorylation and dephosphorylation are both important for multiple steps in the splicing pathway. Members of the PP1 and PP2A subfamilies of phospho-serine/threonine phosphatases play essential but redundant roles in the second step of the splicing reaction. PP6, a member of the PP2A subfamily, is the mammalian homolog of yeast Sit4p and ppe1, which are involved in cell cycle regulation; however, the involvement of PP6 in the splicing pathway remains unclear. Here we show that PP2A family members physically associate with the spliceosome throughout the splicing reaction. PP2A holoenzyme and PP6 were found stably associated with U1 snRNP. Together our findings indicate that these phosphatases regulate splicing catalysis involving U1 snRNP and suggest an important evolutionary conserved role of PP2A family phosphatases in pre-mRNA splicing. |
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| Keywords: | PP2A PP6 Phosphatase snRNPs Pre-mRNA splicing |
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