MicroRNA-490-5p inhibits proliferation of bladder cancer by targeting c-Fos |
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Authors: | Shiqi Li Xianglai XuXin Xu Zhenghui HuJian Wu Yi ZhuHong Chen Yeqing MaoYiwei Lin Jindan LuoXiangyi Zheng Liping Xie |
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Affiliation: | Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang, PR China |
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Abstract: | MicroRNAs (miRNAs) are non-protein-coding sequences that play a crucial role in tumorigenesis by negatively regulating gene expression. Here, we found that miR-490-5p is down-regulated in human bladder cancer tissue and cell lines compared to normal adjacent tissue and a non-malignant cell line. To better characterize the function of miR-490-5p in bladder cancer, we over-expressed miR-490-5p in bladder cancer cell lines with chemically synthesized mimics. Enforced expression of miR-490-5p in bladder cancer cells significantly inhibited the cell proliferation via G1-phase arrest. Further studies found the decreased c-Fos expression at both mRNA and protein levels and Luciferase reporter assays demonstrated that c-Fos is a direct target of miR-490-5p in bladder cancer. These findings indicate miR-490-5p to be a novel tumor suppressor of bladder cancer cell proliferation through targeting c-Fos. |
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Keywords: | miR-490-5p c-Fos Bladder cancer Proliferation |
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