| 氧载体强化氧传递促进法夫酵母虾青素的合成* |
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| 引用本文： | 梁新乐 励建荣 陈敏 张虹. 氧载体强化氧传递促进法夫酵母虾青素的合成*[J]. 菌物学报, 2003, 22(3): 424-429 |
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| 作者姓名： | 梁新乐 励建荣 陈敏 张虹 |
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| 作者单位： | 杭州商学院生物工程系,杭州,310035 |
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| 基金项目： | 浙江省教育厅科研基金项目 |
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| 摘 要： | 法夫酵母生物合成虾青素是强好氧发酵过程,溶氧水平直接影响细胞虾青素的产率。本文对虾青素的氧载体强化氧传递双液相发酵进行了研究。实验结果表明,添加豆油(作为氧载体)可提高法夫酵母发酵时的溶氧水平,促进虾青素的合成:添加豆油 0.5-5.0%(w/v),虾青素产量随豆油添加量逐步提高,最高时达到2.98mg/L,对照组虾青素产率为2.50mg/L。并证明产量的提高是单位质量细胞的虾青素合成效率提高的结果。摇瓶培养时转速的高低不同,对豆油的最适添加量存在影响。较高摇瓶转速有利于豆油在培养基中分散,从而利于强化氧的传递。
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| 关 键 词： | 豆油 类胡萝卜素 氧传递 双液相发酵 |
| 文章编号： | 1007-3515(2003)03-0424-0429 |
| 修稿时间： | 2003-01-02 |
APPLICATION OF OXYGEN VECTORS TO PHAFFIA RHODOZYMA CULTIVATION FOR ASTAXANTHIN PRODUCTION |
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| LIANG Xin-Le LI Jiang-Rong CHEN Ming ZHANG Hong. APPLICATION OF OXYGEN VECTORS TO PHAFFIA RHODOZYMA CULTIVATION FOR ASTAXANTHIN PRODUCTION[J]. Mycosystema, 2003, 22(3): 424-429 |
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| Authors: | LIANG Xin-Le LI Jiang-Rong CHEN Ming ZHANG Hong |
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| Abstract: | Astaxanthin accumulation by Phaffia rhodozyma is strongly dependent on the dissolved oxygen concentration. Two-phase fermentation system with the addition of oxygen vector was applied to enhance oxygen transfer. The addition of oxygen carrier, soybean oil, obviously improved the astaxanthin yield, whereas high concentration of carrier showed somewhat inhibition to the yeast growth and astaxanthin formation. |
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| Keywords: | soybean oil carotenoid oxygen transfer and two-phase fermentation. |
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