Isolation and Characterization of Chitosanase from the Strain Bacillus sp. 739 |
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Authors: | G. E. Aktuganov A. V. Shirokov A. I. Melent'ev |
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Affiliation: | (1) Russian Academy of Sciences, Institute of Biology, Ufa Research Center, Ufa, 450054, Russia |
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Abstract: | The specific nature of the chitosanase activity of the strain Bacillus sp. 739 was determined. Maximum enzyme activity was observed in a medium containing biomass of the fruiting bodies of the fungus Macrolepiota procera. The chitosanase was purified to homogeneity by chromatography on DEAE-Sephadex A-50 and Toyopearl HW-50. The molecular weight of the enzyme assessed by electrophoresis (the Laemmli procedure) approximated 46 kDa. The temperature and pH optima of the purified chitosanase were in the ranges 45–55°C and 6.0–6.5, respectively. Time to half-maximum inactivation of the enzyme at 50°C was equal to 1 h. With colloidal chitosan as the substrate, the value of K of the purified chitosanase was equal to 25 mg/ml. The enzyme also exhibited a weak ability to hydrolyze colloidal chitin. |
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