Chloroplastic and cytoplasmic valyl- and leucyl- tRNA synthetases from Euglena gracilis: Comparative study of their structural properties |
| |
Authors: | Bernard Colas Patrice Imbault Vassilis Sarantoglou Yves Boulanger Jacques-Henry Weil |
| |
Institution: | Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, 15 rue René Descartes, 67084 Strasbourg Cedex France |
| |
Abstract: | Chloroplastic and cytoplasmic valyl- and leucyl-tRNA synthetases purified from Euglena gracilis show a monomeric structure. The molecular weights of the two valyl-tRNA synthetases are identical (126 000) while those of the leucyl-tRNA synthetases are different (100 000 for the chloroplastic and 116 000 for the cytoplasmic enzyme). The tryptic maps and the amino acid compositions reveal differences between the chloroplastic valyl- and leucyl-tRNA synthetases and their cytoplasmic homologues. These results suggest that a chloroplastic aminoacyl-tRNA synthetase and its cytoplasmic counterpart are coded for by distinct genes. |
| |
Keywords: | Valyl-tRNA synthetase Leucyl-tRNA synthetase (E gracilis) |
本文献已被 ScienceDirect 等数据库收录! |