Stabilization of the tertiary structure of yeast phenylalanine tRNA by [Co(NH3)6]3+: X-ray evidence for hydrogen bonding to pairs of guanine bases in the major groove |
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| Authors: | Brian E Hingerty Raymond S Brown Aaron Klug |
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| Institution: | Medical Research Council Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge CB2 2QH U.K. |
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| Abstract: | The sites of three Co(NH3)6]3+ ions bound to the phenylalanine tRNA of yeast have been determined by X-ray diffraction analysis. Co(NH3)6]3+ binds to purine-purine sequences in yeast tRNAPhe. It is different from the binding of Co2+, which binds to the base and phosphate of residue G15. There are no direct metal-nucleotide bonds, although hydrogen bonding of the coordinated ammines to double-helical guanylguanosine sequences in the major groove and to phosphate oxygens in neighboring polynucleotide strands increases the stability of the structure. Hydrogen-bonding appears to be via cis ammine ligands to N(7) and O(6) positions of adjacent purine bases. |
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| Keywords: | X-ray crystallography tRNA stability Hydrogen bond Guanylguanosine Major groove |
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