Normal response of Fanconi's anemia cells to high concentrations of O2 as determined by alkaline elution |
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| Authors: | D.S. Seres A.J. Fornace |
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| Affiliation: | 1. Laboratory of Human Carcinogenesis, Division of Cancer Cause and Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205 U.S.A.;2. Laboratory of Pathology, Division of Cancer Biology and Diagnosis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205 U.S.A. |
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| Abstract: | In this investigation, normal and Fanconi's anemia fibroblasts were exposed to high concentrations of oxygen and the effects of this treatment on DNA were analyzed by alkaline elution. No DNA single-strand breaks were detected in either cell type with up to 20 h incubation in high (50–95%) concentrations of O2. No evidence of DNA damage by O2 could be detected with an endonuclease preparation from Micrococcus luteus. Cells which have been treated with various DNA-damaging agents in the presence of the polymerase inhibitor cytosine arabinoside have been shown to accumulate DNA single-strand breaks during DNA excision repair. When cells were treated with the polymerase inhibitor in 50 or 95% O2, a low level of DNA single-strand breaks accumulated in both cell types. However, no significant differences in the frequency of DNA single-strand breaks were detected between normal and Fanconi's anemia cells after exposure to high O2. |
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| Keywords: | DNA damage DNA repair Oxygen concentration Cytosine arabinoside Alkaline elution (Fanconi's anemia) |
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