Developmental regulation of protein synthesis during Polysphondylium pallidum cyst germination: A two-dimensional gel electrophoresis study |
| |
Authors: | Donald J. Dowbenko Herbert L. Ennis |
| |
Affiliation: | Roche Institute of Molecular Biology, Nutley, NJ 07110 U.S.A. |
| |
Abstract: | Microcyst germination in Polysphondylium pallidum can be used as a model for studying gene expression because temporally regulated modulations in protein synthesis occur in this developmental pathway. Germinating cysts were labeled with [35S]methionine for half-hourly periods during the synchronous germination sequence, and the proteins labeled in each period were resolved by two-dimensional polyacrylamide gel electrophoresis. Three major classes of proteins observed were distinguished by the time of onset and duration of their synthesis: (a) proteins made throughout germination; (b) proteins synthesized only during a portion of the germination pathway; and (c) polypeptides whose synthesis started at 1 or 1.5 h and then continued throughout germination. |
| |
Keywords: | Protein synthesis Cyst germination Development (Polysphondylium pallidium) |
本文献已被 ScienceDirect 等数据库收录! |